Test principle

According to Henderson-Hasselbalch equation (pH = pK + log[HCO₃^-]/[pCO₂]), it can be considered that pH mainly depends on concentration of HCO₃^- when PCO₂ is less than 0.5Kpa. HCO₃^- reacts with phenol red, showing a yellow color and an absorption at 546 nm. The absorbance is linear with the concentration of HCO₃^-, which can be dtermined by comparing with the reference solution.      

Product Features

1.    Linear range of CO₂ level: [0, 50] mmol/L

Linear correlation coefficient ∣r∣≥0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.30  

3.    Sensitivity

The change in absorbance (ΔmA) ≥ 2.00 while testing the specific sample of concentration of 25.0 mmol/L.

4.    Accuracy

Relative deviation is within ±10%.

5.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.

Technical Parameters

Reference range of CO₂ level: 23 – 29 mmol/L.

Packing Specifications

R1: 1×20 ml, R2: 1×20 ml

R1: 1×40 ml, R2: 1×40 ml

R1: 1×60 ml, R2: 1×60 ml

R1: 1×80 ml, R2: 1×80 ml

R1: 2×40 ml, R2: 2×40 ml

R1: 2×80 ml, R2: 2×80 ml

R1: 4×40 ml, R2: 4×40 ml

Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.