Test principle
In moderately acidic solution, Ca react with Arsenazo III, producing purplish-red complex which shows absorption at 650 nm. The intensity of the color is proportional to the concentration of Ca in the specimen.
Product Features
1. Linear range of Ca level: [0.10, 3.75] mmol/L
Linear correlation coefficient ∣r∣≥0.990
Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≤ 1.50
3. Sensitivity
The change in absorbance (ΔA) ≥ 0.10 while testing the specific Ca sample of concentration of 2.46 mmol/L.
4. Accuracy
Relative deviation is within ±10%.
5. Precision
Intra CV% ≤ 5.0%; inter CV% ≤ 6.0%.
Technical Parameters
Temperature |
37℃ |
Optical path length (OPL) |
1.0 cm |
Main wavelength |
650 nm |
Secondary wavelength |
800 nm (Optional) |
Method |
End-point method (single reagent) |
Reaction direction |
Positive |
Sample |
5 μL |
Reagent |
300 μL |
Reaction time |
300 s |
|
|
Reference range of Ca level: 2.17 – 2.57 mmol/L.
Packing Specifications
R1: 1×40 ml, R2: 2×20 ml
R1: 4×40 ml, R2: 8×20 ml
R1: 1×100 ml, R2: 2×50 ml
R1: 2×100 ml, R2: 4×50 ml
R1: 3×100 ml, R2: 6×50 ml
Storage and stability
Unopened: Store test kits at 10 - 30℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).
Opened: Store test kits at 10 - 30℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the test kit.