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Calcium (Ca) Test Kit ( Arsenazo III method)

The Calcium (Ca) test kit is intended for the accurate and direct quantitative measurement of Ca in human plasma.
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Test principle

In moderately acidic solution, Ca react with Arsenazo III, producing purplish-red complex which shows absorption at 650 nm. The intensity of the color is proportional to the concentration of Ca in the specimen. 

 

Product Features

1.    Linear range of Ca level: [0.10, 3.75] mmol/L

Linear correlation coefficient ∣r∣≥0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 1.50  

3.    Sensitivity

The change in absorbance (ΔA) ≥ 0.10 while testing the specific Ca sample of concentration of 2.46 mmol/L.

4.    Accuracy

Relative deviation is within ±10%.

5.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 6.0%.

 

Technical Parameters

Temperature

37

Optical path length (OPL)

1.0 cm

Main wavelength

650 nm

Secondary wavelength

800 nm (Optional)

Method

End-point method

(single reagent)

Reaction direction

Positive

Sample

5 μL

Reagent

300 μL

Reaction time

300 s

Reference range of Ca level: 2.17 – 2.57 mmol/L.


Packing Specifications

R1: 1×40 ml, R2: 2×20 ml

R1: 4×40 ml, R2: 8×20 ml

R1: 1×100 ml, R2: 2×50 ml

R1: 2×100 ml, R2: 4×50 ml

R1: 3×100 ml, R2: 6×50 ml


Storage and stability

Unopened: Store test kits at 10 - 30℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 10 - 30℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.

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