Diagnostic Reagents-Shandong Biomedia Laboratories Co. Ltd.

Lactic Dehydrogenase (LDH) Test Kit (LD-L method)

The lactic dehydrogenase (LDH) test kit is intended for the accurate and direct quantitative measurement of LDH activity in human plasma. Increased LDH levels are usually observed in myocardial infarction, pulmonary infarction, liver disease, blood disease and muscle disease. The LDH test is widely used in the diagnosis of myocardial infraction and liver disease.

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Test principle

Lactic dehydrogenase (LDH) catalyzes the reversible reaction:

The bidirectional reaction is monitored spectrophotometrically by measuring the increase in NADH at 340 nm in the forward reaction, and the increasing rate of absorbance is proportional to LDH activity.

Product Features

1. Linear range of LDH activity: [5, 800] U/L

Linear correlation coefficient ∣r∣≥ 0.990

Linear deviation ≤ ± 10.00%

2. Absorbance of blank ≤ 0.70

3. Changing rate of absorbance of blank ΔA/min ≤ 0.02

4. Sensitivity

Changing rate of absorbance of blank ΔmA/min ≥ 0.03 while testing the specific LDH sample of activity of 256 U/L.

5. Accuracy

Relative deviation is within ±10%.

6. Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.

Technical Parameters

Temperature	37℃	Optical path length (OPL)	1.0 cm
Main wavelength	340 nm	Secondary wavelength	450 nm (Optional)
Method	Velocity method	Reaction direction	Positive
Sample	10 μL	R1	200 μL
R2	50 μL	Delay time	120 s
Reaction time	180 s

Reference range of LDH activity: 110 – 245 U/L.

Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.

Lactic Dehydrogenase (LDH) Test Kit (LD-L method)

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