Test principle

The Creatine Kinase (CK) test kit is based on an enzymatic coupling method. In the reaction, phosphocreatine and ADP are concerted into creatine and ATP. The generated ATP then reacts with glucose catalyzed by hexokinase (HK), resulting in glucose-6-phosphate (G-6-P), which reacts with NADP⁺ to produce NADPH and 6-phospho-D-gluconate.

The concentration of CK can be calculated from the changing rate of absorbance detected at 340 nm.

Product Features

1.    Linear range of CK activity: [5, 1000] U/L

Linear correlation coefficient ∣r∣≥ 0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.70

3.    Changing rate of absorbance of blank ΔA/min ≤ 0.02  

4.    Sensitivity

Changing rate of absorbance of blank ΔmA/min ≥ 0.10 while testing the specific CK sample of activity of 327 U/L.

5.    Accuracy

Relative deviation is within ±10%.

6.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.

Technical Parameters

Reference range of CK activity: 23 – 193 U/L.

Packing Specifications

R1: 1×40 ml, R2: 1×10 ml

R1: 2×40 ml, R2: 1×20 ml

R1: 2×60 ml, R2: 2×15 ml

R1: 2×80 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×15 ml

R1: 3×80 ml, R2: 3×20 ml

Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.