Test principle
The test principle is shown as followings:
There is a strong absorption peak of red quinochrome at 520 nm, and the absorbance is proportional to the concentration of CHOL.
Product Features
1. Linear range of CHOL level: [0.20, 10.40] mmol/L
Linear correlation coefficient ∣r∣≥0.990
Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≤ 0.30
3. Sensitivity
The change in absorbance (ΔA) ≥ 0.01 while testing the specific CHOL sample of concentration of 5.20 mmol/L.
4. Accuracy
Relative deviation is within ±10%.
5. Precision
Intra CV% ≤ 5.0%; inter CV% ≤ 6.0%.
Technical Parameters
Temperature |
37℃ |
Optical path length (OPL) |
1.0 cm |
Main wavelength |
520 nm |
Secondary wavelength |
600 nm (Optional) |
Method |
End-point method (single reagent) |
Reaction direction |
Positive |
Sample |
3 μL |
Reagent |
300 μL |
Reaction time |
500 s |
|
|
Reference range of CHOL level: 3.10 – 6.25 mmol/L.
Packing Specifications
R1: 1×40 ml, R2: 2×20 ml
R1: 4×40 ml, R2: 8×20 ml
R1: 1×100 ml, R2: 2×50 ml
R1: 2×100 ml, R2: 4×50 ml
R1: 3×100 ml, R2: 6×50 ml
Storage and stability
Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the test kit.