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Cholesterol (CHOL) Test Kit (COD-PAP method)

The cholesterol (CHOL) test kit is intended for the accurate and direct quantitative measurement of CHOL in human plasma.
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Test principle

The test principle is shown as followings:

There is a strong absorption peak of red quinochrome at 520 nm, and the absorbance is proportional to the concentration of CHOL.  


Product Features

1.    Linear range of CHOL level: [0.20, 10.40] mmol/L

Linear correlation coefficient ∣r∣≥0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.30  

3.    Sensitivity

The change in absorbance (ΔA) ≥ 0.01 while testing the specific CHOL sample of concentration of 5.20 mmol/L.

4.    Accuracy

Relative deviation is within ±10%.

5.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 6.0%.

      

Technical Parameters

Temperature

37

Optical path length (OPL)

1.0 cm

Main wavelength

520 nm

Secondary wavelength

600 nm (Optional)

Method

End-point method

(single reagent)

Reaction direction

Positive

Sample

3 μL

Reagent

300 μL

Reaction time

500 s

Reference range of CHOL level: 3.10 – 6.25 mmol/L.


Packing Specifications

R1: 1×40 ml, R2: 2×20 ml

R1: 4×40 ml, R2: 8×20 ml

R1: 1×100 ml, R2: 2×50 ml

R1: 2×100 ml, R2: 4×50 ml

R1: 3×100 ml, R2: 6×50 ml


Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 16 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.

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