Test principle

In the coupling reaction above, urea is hydrolyzed in the presence of urease, producing NH₃ and CO₂. And then NH₃ reacts with α-KG, generating glutamic acid. In the meanwhile, NADH is oxidized and converted into NAD⁺.    

The concentration of BUN can be calculated from the decreasing rate of absorbance detected at a wavelength of 340 nm.

Product Features

1.    Linear range of BUN activity: [0.3, 33.7] mmol/L

Linear correlation coefficient ∣r∣≥ 0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≥ 0.90

3.    Changing rate of absorbance of blank ΔA/min ≤ 0.02  

4.    Sensitivity

Changing rate of absorbance of blank ΔmA/min ≥ 0.50 while testing the specific BUN sample of concentration of 16.7 mmol/L.

5.    Accuracy

Relative deviation is within ±10%.

6.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.

Technical Parameters

Reference range of ALT activity: 3.0 – 6.4 mmol/L.

Packing Specifications

R1: 1×40 ml, R2: 1×10 ml

R1: 2×40 ml, R2: 1×20 ml

R1: 2×60 ml, R2: 2×15 ml

R1: 2×80 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×15 ml

R1: 3×80 ml, R2: 3×20 ml

Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.