Shandong Biomedia Laboratories Co.,Ltd.
CH EN
86-531-88796262
Home > Diagnostic Reagents

Total Bilirubin (T-Bil) Test Kit (Nitrite oxidation method)

Total Bilirubin (T-Bil) test kit is applied to measure the concentration of T-Bil in human serum or plasma.
Brochure Download

Testing Principle

In the presence of surfactant and accelerating agents, bilirubin can be oxidized by sodium nitrate and converted into biliverdin, which leads to a decrease in absorbance at 450 nm. Total bilirubin concentrations can be calculated from the absorbance change measured at 450 nm.

   

Product Features

1.    Linear range of T-Bil level: [1, 684] μmol/L

Linear correlation coefficient ∣r∣≥0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.10  

3.    Sensitivity

The change in absorbance (ΔmA) ≥ 0.30 while testing the specific T-Bil sample of concentration of 83.1 μmol/L.

4.    Accuracy

Relative deviation is within ±10%.

5.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤5.0%.


Technical Parameters

Temperature

37

Optical path length (OPL)

1.0 cm

Main wavelength

450 nm

Secondary wavelength

546 nm (Optional)

Method

End-point method

(dual reagents)

Reaction direction

Negative

Sample

8 μL

R1

240 μL

R2

60 μL

Reaction time

300 s

Reference range: 3.42 – 20.50 μmol/L

 

Packing Specifications

R1: 1×40 ml, R2: 1×10 ml

R1: 2×40 ml, R2: 1×20 ml

R1: 2×60 ml, R2: 2×15 ml

R1: 2×80 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×15 ml

R1: 3×80 ml, R2: 3×20 ml


Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.

← Go back to the last page
Shandong Biomedia Laboratories Co., Ltd. copyright 2015 Lu ICP No. 12031366 -1
Address: Block B south building,6th floor,Huayang Road #65,Lixia District,Jinan City,Shandong Province,PR. China.Tel:86-531-88796262 Fax:86-531-88796464