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Aspartate Aminotransferase (AST) Test Kit (IFCC method)

The aspartate aminotransferase (AST) test kit is intended for the accurate and direct quantitative measurement of AST activity in human plasma. AST measurement is useful in the diagnosis of liver disease and heart disease.
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Test principle

L- Aspartic acid+α-Ketoglutaric acid AST Oxaloacetic acid+L-Glutamate

L-Glutamate+ NADH+H+ MDH L-Malic acid+NAD+

In the coupling reaction above, the oxidation rate of NADH is proportional to the activity of AST. The unit activity of AST can be calculated from the decreasing rate of absorbance detected at a wavelength of 340 nm.   

Product Features

1.    Linear range of AST activity: [3, 500] U/L

Linear correlation coefficient ∣r∣≥ 0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≥ 0.90

3.    Changing rate of absorbance of blank ΔA/min ≤ 0.02  

4.    Sensitivity

Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific AST sample of activity of 109 U/L.

5.    Accuracy

Relative deviation is within ±10%.

6.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤8.0%.

Technical Parameters



Optical path length (OPL)

1.0 cm

Main wavelength

340 nm

Secondary wavelength

450 nm (Optional)


Velocity method

Reaction direction



12 μL


200 μL


50 μL

Delay time

90 s

Reaction time

250 s

Reference range of AST activity: 11 – 34 U/L.


Packing Specifications

R1: 1×40 ml, R2: 1×10 ml

R1: 2×40 ml, R2: 1×20 ml

R1: 2×60 ml, R2: 2×15 ml

R1: 2×80 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×15 ml

R1: 3×80 ml, R2: 3×20 ml

Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.

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