Test principle

4-Nitrophenyl Phosphate (4-NPP) can be dephosphorylated by ALP and converted into 4- Nitrophenol (4-NP), which turns yellow due to it quinoid structure formed in alkaline environment. The unit activity of ALP can be calculated from the increasing rate of absorbance detected at a wavelength of 405 nm.

Product Features

1.    Linear range of ALP activity: [3, 500] U/L

Linear correlation coefficient ∣r∣≥ 0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.80

3.    Changing rate of absorbance of blank ΔA/min ≤ 0.05  

4.    Sensitivity

Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific ALP sample of activity of 223 U/L.

5.    Accuracy

Relative deviation is within ±10%.

6.    Precision

Intra CV% ≤ 6.0%; inter CV% ≤8.0%.

Technical Parameters

Reference range of ALP activity: 3 – 110 U/L.

Packing Specifications

R1: 1×40 ml, R2: 1×10 ml

R1: 2×40 ml, R2: 1×20 ml

R1: 2×60 ml, R2: 2×15 ml

R1: 2×80 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×15 ml

R1: 3×80 ml, R2: 3×20 ml

Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.