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Total Protein (TP) Test Kit (Biuret method)

The total protein (TP) test kit is intended for the accurate and direct quantitative measurement of TP in human plasma. TP assay is clinically significant in diagnosis of many diseases. Liver protein synthesis disorder and kidney lesion can lead to protein loss and malnutrition, and thus the decrease of TP level, while cirrhosis and water deprivation can result in a remarkable increase.
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Test principle

In alkaline solution, peptide bonds in protein combine with Cu2+, generating blue violet compounds. The absorbance of these compounds at 550 nm is proportional to the quantity of peptide bonds, and thus concentration of total protein can be calculated from the measured absorbance.

Product Features

1.    Linear range of TP level: [5, 90] g/L

Linear correlation coefficient ∣r∣≥0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.30  

3.    Sensitivity

The change in absorbance (ΔmA) ≥ 0.50 while testing the specific TP sample of concentration of 48.9 g/L.

4.    Accuracy

Relative deviation is within ±10%.

5.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.

Technical Parameters



Optical path length (OPL)

1.0 cm

Main wavelength

550 nm

Secondary wavelength

700 nm (Optional)


End-point method

(single reagent)

Reaction direction



5 μL


300 μL

Reaction time

300 s

Reference range of TP level: 60 – 83 g/L.

Packing Specifications

R1: 1×40 ml, R2: 2×20 ml

R1: 4×40 ml, R2: 8×20 ml

R1: 1×100 ml, R2: 2×50 ml

R1: 2×100 ml, R2: 4×50 ml

R1: 3×100 ml, R2: 6×50 ml


Storage and stability

Unopened: Store test kits at 10 - 30℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 10 - 30℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.

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