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γ-Glutamyltransferase (GGT) Test Kit (Velocity method)

The γ-Glutamyltransferase (GGT) test kit is intended for the accurate and direct quantitative measurement of GGT activity in human plasma. GGT measurement is useful in the diagnosis of hepatobiliary disease.
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Test principle

In this test kit, soluble L-γ-glutamyl-3-carboxyl-p-nitroaniline (Glupac) serves as a substrate, and glycyglycine is designed to be a receptor of γ-glutamyl. γ-glutamyl can be transferred to glycyglycine molecules when catalyzed by GGT, producing yellow-colored 3-hydroxyl-p-nitroaniline. The reaction can induce an increase of absorbance at 405 nm, and the increasing rate is proportional to the activity of GGT.


Product Features

1.    Linear range of GGT activity: [2, 500] U/L

Linear correlation coefficient ∣r∣≥ 0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.90

3.    Changing rate of absorbance of blank ΔA/min ≤ 0.05  

4.    Sensitivity

Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific GGT sample of activity of 100 U/L.

5.    Accuracy

Relative deviation is within ±10%.

6.    Precision

Intra CV% ≤ 6.0%; inter CV% ≤8.0%.     

Technical Parameters



Optical path length (OPL)

1.0 cm

Main wavelength

405 nm

Secondary wavelength

480 nm (Optional)


Velocity method

Reaction direction



10 μL


200 μL


50 μL

Delay time

100 s

Reaction time

180 s

Reference range of GGT activity: 5 – 50 U/L.


Packing Specifications

R1: 1×40 ml, R2: 1×10 ml

R1: 2×40 ml, R2: 1×20 ml

R1: 2×60 ml, R2: 2×15 ml

R1: 2×80 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×15 ml

R1: 3×80 ml, R2: 3×20 ml

Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.

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