Testing Principle

This test kit is applied to test the HDL-C levels based on PPD (polyanion polymer/ detergent) method. During the reaction, LDL, VLDL and chylomicrons are suppressed by the polyanion polymer and detergent and can not react with cholesterol oxidase reagent. In the meanwhile, HDL-C is dissolved by the surfactant, resulting in the release of cholesterol, and then the enzymes react with HDL. The concentration of HDL can be calculated by the change of absorbance of the serum sample.   

Product Features

Linearity range: 0.50 – 3.90 mmol/L; linear correlation coefficient ≥0.990; linearity deviation is within ±10%.

Absorbance of blank≤ 0.10

Sensitivity: the difference of absorbance per unit of concentration (ΔA) ≥ 0.01 while testing the specific sample of concentration of 1.87 mmol/L.

Accuracy: Relative deviation is within ±10%.

Precision: intra assay CV(%) ≤ 5%; inter assay CV(%)  ≤ 5%.

Technical Parameters

Reference range: 1.19 – 1.69 mmol/L

Packaging Specification

R1: 1×30 ml, R2: 1×10 ml

R1: 2×30 ml, R2: 1×20 ml

R1: 2×45 ml, R2: 2×15 ml

R1: 2×60 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×20 ml

R1: 3×80 ml, R2: 4×20 ml

Calibrator: additional purchase

Quality control: additional purchase

Storage and Period of Validity

Store the Biomedia HDL-C test kits at 2 to 8 ℃ protect from light. The period of validity of unopened reagents can be up to 16 months. Opened reagents can be valid for 1 month if stored at 2 to 8 ℃ protect from light.