Testing Principle
The Biomedia Hcy test kit is based on latex immunoturbidimetric method.
During the assay, Cys-C in human serum reacts with antibody absorbed on latex particles, forming antigen-antibody complex. The concentration of Cys-C can be calculated from the absorbance changes, which reflect the change of turbidity.
Product Features
1. Linear range
a. Linear correlation coefficient ∣r∣≥0.990
b. Linear range of Cys-C level: [0.40, 7.50] mg/L
2. Absorbance of blank ≤ 1.20
3. Sensitivity
The difference of absorbance (ΔA) ≥ 0.04 while testing the specific Cys-C sample of concentration of 1.00 mg/L.
4. Accuracy
Check the accuracy using the following methods:
(a) Relative deviation is within ±15%.
(b) In contrast experiment, the absolute deviation is within ±20%, and the Linear correlation coefficient is no less than 0.975.
5. Precision
Intra CV% ≤ 5.0%;inter CV% ≤ 10.0%.
Technical Parameters
Reference range: 0.00 – 1.25 mg/L
Packaging Specification
R1: 1×35 ml, R2: 1×7 ml
R1: 1×50 ml, R2: 1×10 ml
R1: 2×50 ml, R2: 2×10 ml
R1: 4×50 ml, R2: 2×20 ml
R1: 6×50 ml, R2: 3×20 ml
Storage and Period of Validity
Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 12 months).
Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 months if kept capped from original container and free from contamination.