Testing Principle

In the coupling reaction above, the oxidation rate of NADH is proportional to the activity of ALT. The unit activity of ALT can be calculated from the decreasing rate of absorbance detected at a wavelength of 304 nm.

Product Features

Linearity range: 5 – 500 U/L; linear correlation coefficient ≥0.990; linearity deviation is within ±10%.

Absorbance of blank ≥ 0.90

Changing rate of absorbance of blank (ΔA/min) ≤ 0.05

Sensitivity: the difference of absorbance per unit of activity (ΔA) ≥ 0.05 while testing the specific sample of 110 U/L.

Accuracy: Relative deviation is within ±10%.

Precision:  intra assay CV(%) ≤ 6%; inter assay CV(%)  ≤ 8%.

Technical Parameters

Reference range: 6 – 40 U/L

Available Instrument

The Biomedia ALT test kit is applicable to ALT measurement using automatic biochemical analyzer.

Packaging Specification

R1: 2×60 ml, R2: 2×15 ml

R1: 4×60 ml, R2: 4×15 ml

Storage and Period of Validity

Store the Biomedia ALT test kits at 2 to 8 ℃ protected from light. The period of validity of unopened reagents can be up to 15 months. Opened reagents can be valid for 1 month if stored at 2 to 8 ℃ protected from light.