Testing Principle

During the assay, CRE is converted to creatine by creatininase. Creatine is converted to sarcosine in the presence of creatinase. Sarcosine is then oxidized, and the product reacts with the probe, generating red quinochrome, concentration of which can be determined by the colorimetric method. The concentration of CRE can be calculated from that of red quinochrome.

Product Features

Linearity range: 2 – 880 μmol/L; linear correlation coefficient ≥0.990; linearity deviation is within ±10%.

Absorbance of blank ≤ 0.50

Sensitivity: the difference of absorbance per unit of concentration (ΔA) ≥ 0.10 while testing the specific sample of concentration of 442 μmol/L.

Accuracy: Relative deviation is within ±10%.

Precision: CV ≤ 5%; precision between batches ≤ 5%.

Technical Parameters

Reference range: 54 – 97 μmol/L

Packaging Specification

R1: 1×30 ml, R2: 1×10 ml

R1: 2×30 ml, R2: 1×20 ml

R1: 2×45 ml, R2: 2×15 ml

R1: 2×60 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×20 ml

R1: 3×80 ml, R2: 4×20 ml

Calibrator: appendant

Quality control: additional purchase

Storage and Period of Validity

Store the Biomedia CRE test kits at 2 to 8 ℃ protected from light. The period of validity of unopened reagents can be up to 16 months. Opened reagents can be valid for 1 month if stored at 2 to 8 ℃ protected from light.